top of page

20Med PNP platform

Ready-to-use delivery platform for intracellular delivery of nucleotide-based payloads. 

20Med PNP composition

20Med nanoparticles are composed of bio-responsive poly(amido)amines (PAA) specifically modified for efficient intracellular delivery of nucleotide-based payloads such as oligonucleotides and mRNA or DNA. The polymers provide enhanced stability and increased biological effectivity to the system.

20Med nanoparticles are formed when the polymeric components are combined with the nucleotide payload in a single and highly efficient formulation step. Solvents are not used for the formulation of the polymers with the payload which leads to a maximum yield since additional purification steps are not needed.

Cellular uptake of 20Med particle, endosomal escape of the 20Med nanoparticles, bioresponsive degradation of the polymeric nanoparticles and release of payload into the cytosol

20Med PNP technology

20Med's proprietary bio-responsive polymeric nanoparticles provide protection of fragile payloads after administration but allow for rapid bio-responsive release of that payload as soon as the particles enter the cytosol.

  • Cellular uptake - 20Med nanoparticles interact with the cellular membrane and are taken up into the cells via endocytosis.

  • Endosomal escape - in order to deliver the cargo successfully to the nucleus, the 20Med Nanoparticles escape from the endosome by a process called the "proton sponge effect".

  • Bioresponsive degradation - after endosomal escape, the 20Med nanoparticles are located in the cytoplasm. The glutathione in the cytosol breaks down the disulfide bonds in the polymers and enable release of the payload into the cytosol.

  • Release of payload into cytosol

Key Publications

Biomaterials Advances

A poly(amidoamine)-based polymeric nanoparticle platform for efficient in vivo delivery of mRNA

Front. Bioeng. Biotechnol.

Cell uptake and intracellular trafficking of bioreducible poly(amidoamine) nanoparticles for efficient mRNA translation in chondrocytes

Delivery technology_edited.png
bottom of page